1SUO
Structure of mammalian cytochrome P450 2B4 with bound 4-(4-chlorophenyl)imidazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0001 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 233.386, 233.386, 56.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.000 - 1.900 |
| Rwork | 0.215 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1po5 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.074 | 0.553 |
| Number of reflections | 638636 | |
| <I/σ(I)> | 6.8 | 1.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | NACL, PEG 10,000, PHOSPHATE-CITRATE PH 4.0, GLYCEROL, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K |
