1SU8
Carbon Monoxide Induced Decomposition of the Active Site [Ni-4Fe-5S] Cluster of CO Dehydrogenase
Replaces: 1JJYExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.05 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.792, 74.740, 70.930 |
| Unit cell angles | 90.00, 111.16, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.100 |
| R-factor | 0.1463 |
| Rwork | 0.146 |
| R-free | 0.18490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.100 |
| Number of reflections | 216577 |
| Completeness [%] | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 3000, 2-Propanol, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
