1SU5
Understanding protein lids: Structural analysis of active hinge mutants in triosephosphate isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.756, 86.756, 163.376 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.790 - 2.700 |
| R-factor | 0.18726 |
| Rwork | 0.185 |
| R-free | 0.23638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8tim |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.235 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.590 |
| Rmerge | 0.109 | 0.392 |
| Number of reflections | 19906 | |
| <I/σ(I)> | 16.5 | 5.2 |
| Completeness [%] | 99.0 | 97.5 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | Citrate, ammonium sulphate, sodium chloride, 2-phosphoglycolate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
