1SU0
Crystal structure of a hypothetical protein at 2.3 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9794, 0.9796, 0.9600 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 72.014, 72.014, 112.713 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.2215 |
Rwork | 0.220 |
R-free | 0.25582 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.362 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.359 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 6915 | |
Completeness [%] | 98.0 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.9 M Sodium Malonate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |