1STS

STREPTAVIDIN DIMERIZED BY DISULFIDE-BONDED PEPTIDE FCHPQNT-NH2 DIMER

Experimental procedure

Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	I 2 2 2
Unit cell lengths	97.840, 105.790, 49.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.950
R-factor	0.191
Rwork	0.191
R-free	0.23800
RMSD bond length	0.019
RMSD bond angle	27.600 *
Data reduction software	SADIE
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.740
Rmerge	0.089
Total number of observations	46268 *
Number of reflections	20222
Completeness [%]	72.2
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	30-32 (%sat)
2	1	reservoir	potassium acetate	0.1 (M)