1STP
STRUCTURAL ORIGINS OF HIGH-AFFINITY BIOTIN BINDING TO STREPTAVIDIN
Experimental procedure
Spacegroup name | I 41 2 2 |
Unit cell lengths | 99.400, 99.400, 125.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 * - 2.600 |
R-factor | 0.22 |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.700 * |
Rmerge | 0.052 * |
Total number of observations | 89393 * |
Number of reflections | 13496 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 9-13 (%(w/v)) | |
2 | 1 | reservoir | citric acid-sodium phsophate | in pH6.0-8.0 | |
3 | 1 | reservoir | 1 (M) | can be substituted with 2 M NaCl or KCl |