1STD

CRYSTAL STRUCTURE OF SCYTALONE DEHYDRATASE: A DISEASE DETERMINANT OF THE RICE PATHOGEN, MAGNAPORTHE GRISEA

Experimental procedure

Spacegroup name	P 3 2 1
Unit cell lengths	75.500, 75.500, 73.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 * - 2.900
R-factor	0.191 *
Rwork	0.179
RMSD bond length	0.012
RMSD bond angle	3.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.900 *
Rmerge	0.085 *
Total number of observations	41844 *
Number of reflections	5646 *
Completeness [%]	99.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *	Lundqvist, T., (1993) J.Mol.Biol., 232, 999. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	acetate	0.2 (M)
2	1	reservoir	ammonium sulfate	0.28 (M)
3	1	reservoir	PEG4000	10-15 (%)
4	1	drop	protein	8 (mg/ml)