1STA
ACCOMMODATION OF INSERTION MUTATIONS ON THE SURFACE AND IN THE INTERIOR OF STAPHYLOCOCCAL NUCLEASE
Experimental procedure
| Spacegroup name | P 41 |
| Unit cell lengths | 47.896, 47.896, 62.998 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.690 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 10.5 (mM) | |
| 3 | 1 | drop | MPD | 17 (%) | |
| 4 | 1 | drop | pdTp | 2 (mg/ml) | |
| 5 | 1 | drop | 10 (mM) | ||
| 6 | 1 | drop | potassium citrate | 20 (mM) | |
| 7 | 1 | reservoir | MPD | 21-23 (%(w/w)) | |
| 8 | 1 | reservoir | potassium phosphate | 10.5 (mM) |
