1ST6

Crystal structure of a cytoskeletal protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-06-07
Detector	MARRESEARCH
Wavelength(s)	0.93924, 0.97955
Spacegroup name	C 2 2 21
Unit cell lengths	56.020, 126.947, 351.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.960 - 3.100
R-factor	0.316
Rwork	0.316
R-free	0.35700
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	2.300
Data reduction software	DENZO
Phasing software	SHELXD
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.960	3.290
High resolution limit [Å]	3.100	3.100
Rmerge	0.092
Number of reflections	23379
<I/σ(I)>	15.1
Completeness [%]	99.9	99.7
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	303	ammonium sulphate, cacodylic acid, dtt, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 303K