1ST6
Crystal structure of a cytoskeletal protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-06-07 |
Detector | MARRESEARCH |
Wavelength(s) | 0.93924, 0.97955 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 56.020, 126.947, 351.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.960 - 3.100 |
R-factor | 0.316 |
Rwork | 0.316 |
R-free | 0.35700 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 2.300 |
Data reduction software | DENZO |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.960 | 3.290 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.092 | |
Number of reflections | 23379 | |
<I/σ(I)> | 15.1 | |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 303 | ammonium sulphate, cacodylic acid, dtt, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 303K |