1SST
Serine Acetyltransferase- Complex with CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 125 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-25 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 111.300, 125.000, 103.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.250 - 2.000 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ssm |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.250 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.029 | 0.153 |
| Number of reflections | 44011 | |
| Completeness [%] | 90.2 | 88.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 30% (v/v) polyethylene glycol 400, 130 mM LiCl, 50 mM CoA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
