1SSM
Serine Acetyltransferase- Apoenzyme (truncated)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 125 |
Detector technology | CCD |
Collection date | 2002-05-30 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 107.100, 126.600, 107.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.740 - 2.150 |
R-factor | 0.23 |
Rwork | 0.227 |
R-free | 0.27800 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 2.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.700 | 2.280 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.076 | 0.267 |
Number of reflections | 136283 | |
Completeness [%] | 0.9 | 76.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100 mM ADA, 1-4% MPD, 190 mM Li2SO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |