1SSA

A STRUCTURAL INVESTIGATION OF CATALYTICALLY MODIFIED F12OL AND F12OY SEMISYNTHETIC RIBONUCLEASES

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	67.250, 67.250, 64.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 2.000
R-factor	0.161
Rwork	0.161
RMSD bond length	0.025
RMSD bond angle	1.700 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	5.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.065 *
Number of reflections	9078 *
Completeness [%]	80.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	macro seeding *	5.7 *		THE MOTHER LIQUOR WAS 80 % SATURATED AMMONIUM SULFATE, PH 5.2.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNase1-118	0.95 (mM)
2	1	drop	RNase111-124	9.9 (mM)
3	1	reservoir		3.0 (M)
4	1	reservoir		28 (%sat)
5	1	reservoir	ammonium acetate	0.1 (M)
6	1	reservoir	1,10-phenanthroline	5 (mM)