1SRY
REFINED CRYSTAL STRUCTURE OF THE SERYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS AT 2.5 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.030, 127.460, 63.370 |
| Unit cell angles | 90.00, 108.90, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| Rwork | 0.184 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.049 |
| Refinement software | GROMOS |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.060 * |
| Total number of observations | 234441 * |
| Number of reflections | 44704 * |
| Completeness [%] | 97.0 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 20 (%sat) | |
| 3 | 1 | drop | MES | 50 (mM) | |
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | 10 (mM) | ||
| 6 | 1 | drop | 1 (mM) | ||
| 7 | 1 | drop | MPD | 1 (%(v/v)) | |
| 8 | 1 | reservoir | ammonium sulfate | 30-35 (%sat) | |
| 9 | 1 | reservoir | MPD | 8 (%) |
