1SRQ
Crystal Structure of the Rap1GAP catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 170.700, 224.500, 48.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.990 - 2.900 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.27600 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.813 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.972 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.053 | 0.336 |
| Number of reflections | 42290 | |
| <I/σ(I)> | 17.2 | 3.86 |
| Completeness [%] | 98.9 | 99.8 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 277 | PEG 3350, MPD, magnesium sulfate, HEPES, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
