1SR0
Crystal structure of signalling protein from sheep(SPS-40) at 3.0A resolution using crystal grown in the presence of polysaccharides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5412 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.793, 66.386, 107.896 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.000 - 3.050 |
| R-factor | 0.17403 |
| Rwork | 0.172 |
| R-free | 0.20547 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1owq |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.426 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 7210 | |
| <I/σ(I)> | 6.8 | 2.1 |
| Completeness [%] | 80.0 | 78 |
| Redundancy | 28.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 25mM tris-HCl,50mM NACl,19% ethanol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
