1SQV

Crystal Structure Analysis of Bovine Bc1 with UHDBT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-08
Detector	ADSC QUANTUM 4
Wavelength(s)	1.2
Spacegroup name	I 41 2 2
Unit cell lengths	153.672, 153.672, 589.219
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.850
R-factor	0.2191
Rwork	0.217
R-free	0.28495
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.010
RMSD bond angle	1.870
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.850
Number of reflections	68441

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.2	277	20mM ammonium acetate, 20% glycerol, 12% PEG4000, 0.5M KCl, 0.1% diheptanoyl-phosphatidylcholine, pH 7.2, VAPOR DIFFUSION, temperature 277K