1SQP

Crystal Structure Analysis of Bovine Bc1 with Myxothiazol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-02
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	153.700, 153.700, 596.534
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.700
R-factor	0.26447
Rwork	0.263
R-free	0.31379
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1qcr
RMSD bond length	0.021
RMSD bond angle	1.752
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.700
Number of reflections	89603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.2	277	20mM ammonium acetate, 20% glycerol, 12% PEG4000, 0.5M KCl, 0.1% diheptanoyl-phosphatidylcholine, pH 7.2, VAPOR DIFFUSION, temperature 277K