1SQH
X-RAY STRUCTURE OF DROSOPHILA MALONOGASTER PROTEIN Q9VR51 NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET FR87.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-01-31 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | H 3 2 |
Unit cell lengths | 82.806, 82.806, 279.003 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.840 - 2.000 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.26200 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.109 | |
Number of reflections | 43215 | |
Completeness [%] | 88.8 | 49.7 |
Redundancy | 3.25 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 277 | 100 mM ammonium sulfate, 100 mM Tris, Spermidine , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 7.00 |