1SQ3
Crystal structures of a novel open pore ferritin from the hyperthermophilic Archaeon Archaeoglobus fulgidus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-27 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 184.502, 190.243, 179.407 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.17877 |
| Rwork | 0.177 |
| R-free | 0.21755 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s3q |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.461 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.097 | 0.394 |
| Number of reflections | 86172 | |
| <I/σ(I)> | 26.43 | 6.67 |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 7.5 | 293 | PEG 400, HEPES, MgCl2, FeSO4 , pH 7.5, MICROBATCH UNDER OIL, temperature 293K |
