1SPR

BINDING OF A HIGH AFFINITY PHOSPHOTYROSYL PEPTIDE TO THE SRC SH2 DOMAIN: CRYSTAL STRUCTURES OF THE COMPLEXED AND PEPTIDE-FREE FORMS

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	110.600, 86.200, 58.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.500
R-factor	0.185
Rwork	0.185
RMSD bond length	0.014
RMSD bond angle	3.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.460 *
Rmerge	0.068 *
Total number of observations	52494 *
Number of reflections	19669 *
Completeness [%]	89.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000
2	1	reservoir		200 (mM)