1SOX

SULFITE OXIDASE FROM CHICKEN LIVER

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-08-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	55.117, 197.690, 56.009
Unit cell angles	90.00, 94.23, 90.00

Refinement procedure

Resolution	50.000 - 1.900
R-factor	0.1748
Rwork	0.175
R-free	0.22020 *
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.060 *	0.160 *
Number of reflections	84147
<I/σ(I)>	11.4
Completeness [%]	89.2	66.2
Redundancy	2.1	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	22 *	used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir		0.65-0.9 (M)
3	1	reservoir	HEPES	100 (mM)	pH7.5