1SOV
Toxoplasma gondii bradyzoite-specific LDH (LDH2) apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-12-18 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 142.939, 142.939, 164.518 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.173 |
Rwork | 0.173 |
R-free | 0.20100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pze |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.053 | 0.322 |
Number of reflections | 66552 | |
<I/σ(I)> | 29 | 4.9 |
Completeness [%] | 99.2 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.75 | 295 | 1.5 M ammonium phosphate, 100 mM imidazole, pH 7.75, VAPOR DIFFUSION, HANGING DROP, temperature 295K |