1SOS
ATOMIC STRUCTURES OF WILD-TYPE AND THERMOSTABLE MUTANT RECOMBINANT HUMAN CU, ZN SUPEROXIDE DISMUTASE
Experimental procedure
Spacegroup name | C 2 2 21 |
Unit cell lengths | 205.200, 167.000, 145.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 * - 2.500 |
Rwork | 0.202 |
RMSD bond length | 0.017 |
RMSD bond angle | 3.500 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.480 * |
Total number of observations | 742579 * |
Number of reflections | 77344 * |
Completeness [%] | 88.4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | potassium phosphate | 50 (mM) | |
2 | 1 | drop | protein | 2 (%(w/v)) | |
3 | 1 | reservoir | Tris-HCl | 50 (mM) | |
4 | 1 | reservoir | 50 (mM) | ||
5 | 1 | reservoir | EDTA | 0.1 (mM) | |
6 | 1 | reservoir | 0.01 (%) |