1SOQ
Crystal structure of the transthyretin mutant A108Y/L110E solved in space group C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAC Science DIP-2030 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.287, 67.524, 80.978 |
| Unit cell angles | 90.00, 112.59, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sok |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.251 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.077 | 0.407 |
| Number of reflections | 29735 | |
| <I/σ(I)> | 13.8 | 2.7 |
| Completeness [%] | 95.4 | 92.7 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 291 | Tris, PEG550MME, ammonium sulfate, sodium citrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 6.00 |
