1SNX
CRYSTAL STRUCTURE OF APO INTERLEUKIN-2 TYROSINE KINASE CATALYTIC DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-26 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.500, 54.900, 59.200 |
| Unit cell angles | 92.50, 85.50, 74.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.252 |
| Rwork | 0.241 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Homology model based on BTK |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.082 | 0.345 |
| Number of reflections | 9901 | |
| <I/σ(I)> | 10.5 | 2.1 |
| Completeness [%] | 86.4 | 81.4 |
| Redundancy | 1.5 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | Peg 3350, Ammonium Acetate, MES, DTT, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
