1SNO

PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE

Experimental procedure

Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1993-07
Detector	RIGAKU
Spacegroup name	P 41
Unit cell lengths	47.740, 47.740, 63.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.700
R-factor	0.17
Rwork	0.170
R-free	0.22400
RMSD bond length	0.009 *
RMSD bond angle	1.693 *
Data reduction software	R-AXIS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.700 *	1.700 *
Rmerge	0.059
Total number of observations	91144 *
Number of reflections	15599
Completeness [%]	98.8	97.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.15	4 *	Loll, P.J., (1989) Proteins: Struct.,Funct., Genet., 5, 183. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	potassium phosphate	10.5 (mM)
3	1	drop	MPD	17 (%(w/w))
4	1	drop		10 (mM)
5	1	drop	potassium citrate	20 (mM)
6	1	reservoir	potassium phosphate	10.5 (mM)
7	1	reservoir	MPD	20-30 (%(w/w))