1SNM

ACTIVE SITE MUTANT GLU-43 (RIGHT ARROW) ASP IN STAPHYLOCOCCAL NUCLEASE DISPLAYS NONLOCAL STRUCTURAL CHANGES

Experimental procedure

Spacegroup name	P 41
Unit cell lengths	48.590, 48.590, 63.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.740
R-factor	0.174
RMSD bond length	0.025
RMSD bond angle	0.065
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	12.000 *
High resolution limit [Å]	1.740 *
Rmerge	0.051 *
Completeness [%]	80.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.15 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25.5 (mg/ml)
2	1	drop	MPD	22.6 (%(w/w))
3	1	drop	potassium phosphate	10.5 (mM)