1SNC

THE CRYSTAL STRUCTURE OF THE TERNARY COMPLEX OF STAPHYLOCOCCAL NUCLEASE, CA2+, AND THE INHIBITOR PD*TP, REFINED AT 1.65 ANGSTROMS

Experimental procedure

Spacegroup name	P 41
Unit cell lengths	48.000, 48.000, 63.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.650
R-factor	0.161
RMSD bond length	0.017
RMSD bond angle	0.037
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.600 *	1.650 *
Rmerge	0.025 *	0.022 *
Number of reflections	11332 *
Completeness [%]	61.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	potassium phosphate	10.5 (mM)
3	1	drop	MPD	17 (%(w/w))
4	1	drop		10 (mM)
5	1	drop	potassium citrate	20 (mM)
6	1	reservoir	potassium phosphate	10.5 (mM)
7	1	reservoir	MPD	21-23 (%(w/w))