1SN8

Crystal structure of the S1 domain of RNase E from E. coli (Pb derivative)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.947390
Spacegroup name	P 41 21 2
Unit cell lengths	70.580, 70.580, 87.902
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.000
R-factor	0.18485
Rwork	0.182
R-free	0.23223
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.417
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	15387
<I/σ(I)>	17.5	2.6
Completeness [%]	98.5	86.7
Redundancy	6.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	PEG 8000, sodium cacodylate, sodium acetate, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K