1SN7
KUMAMOLISIN-AS, APOENZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.810, 44.950, 49.110 |
| Unit cell angles | 113.90, 106.10, 102.30 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.1492 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gt9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.033 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 17755 | |
| <I/σ(I)> | 25.8 | 6.7 |
| Completeness [%] | 90.0 | 61.6 |
| Redundancy | 1.6 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | PEG8000, Ammounium Sulfate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
