1SN2
Crystal Structure of Sea Bream Transthyretin at 1.90A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Spacegroup name | P 41 |
| Unit cell lengths | 58.497, 58.497, 140.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.840 - 1.750 |
| R-factor | 0.17836 |
| Rwork | 0.175 |
| R-free | 0.20964 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Human transthyretin pdb code 1F41 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.303 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.047 | 0.451 |
| Number of reflections | 46104 | |
| Completeness [%] | 97.1 | 92.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 296 | PEG 1550 MME, sodium citrate, ammonium sulphate, nickel sulphate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
