1SN0
Crystal Structure Of Sea Bream Transthyretin in complex with thyroxine At 1.9A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Spacegroup name | P 41 |
| Unit cell lengths | 58.455, 58.455, 140.116 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.840 - 1.900 |
| R-factor | 0.1829 |
| Rwork | 0.179 |
| R-free | 0.21630 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.404 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.047 | 0.446 |
| Number of reflections | 35460 | |
| Completeness [%] | 96.0 | 89.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 296 | PEG 1550 MME, sodium citrate, ammonium sulphate, nickel sulphate. Crystals were soaked in thyroxine, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
