1SMX
Crystal structure of the S1 domain of RNase E from E. coli (native)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0781 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 70.378, 70.378, 87.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.20009 |
Rwork | 0.199 |
R-free | 0.23109 |
Structure solution method | SAD |
Starting model (for MR) | Pb derivative structure |
RMSD bond length | 0.013 |
RMSD bond angle | 1.315 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 20912 | |
<I/σ(I)> | 30.8 | 4.65 |
Completeness [%] | 99.0 | 93.5 |
Redundancy | 7.76 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG 8000, sodium cacodylate, sodium acetate, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |