1SML
METALLO BETA LACTAMASE L1 FROM STENOTROPHOMONAS MALTOPHILIA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9090,0.9200,1.2835, 1.2821,0.9801,0.9797 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 105.230, 105.230, 97.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.18 * |
| Rwork | 0.180 |
| R-free | 0.23300 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.700 * |
| Rmerge | 0.055 * |
| Number of reflections | 35028 |
| Completeness [%] | 98.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * | pH 7.75 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
| 6 | 1 | reservoir | HEPES | 100 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 8 | 1 | reservoir | PEG400 | 1.5 (%(v/v)) |
