1SMF

Studies on an artificial trypsin inhibitor peptide derived from the mung bean inhibitor

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	63.230, 63.620, 69.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 * - 2.200 *
R-factor	0.193 *
Rwork	0.190
RMSD bond length	0.014
RMSD bond angle	2.710
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.077 *
Total number of observations	42768 *
Number of reflections	12563 *
Completeness [%]	82.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.4 *	24 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	2 (M)
2	1	drop	phosphate	0.2 (M)
3	1	reservoir	ammonium sulfate	2 (M)
4	1	reservoir	phosphate	0.2 (M)