1SMD

HUMAN SALIVARY AMYLASE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	53.200, 75.400, 136.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.600
R-factor	0.184 *
Rwork	0.184
RMSD bond length	0.010
RMSD bond angle	0.025 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	1.600 *	1.600 *
Rmerge	0.076 *
Completeness [%]	93.4 *	88.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9 *		Ramasubbu, N., (1991) Proteins: Struct.,Funct., Genet., 11, 230. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	20-30 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop		5 (mM)
4	1	reservoir	MPD	26-28 (%)
5	1	reservoir	water