1SMB
Crystal Structure of Golgi-Associated PR-1 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 73.662, 73.662, 63.363 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.490 - 1.550 |
| Rwork | 0.188 |
| R-free | 0.19680 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.513 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.077 | 0.278 |
| Number of reflections | 28674 | |
| <I/σ(I)> | 6.2 | 2.5 |
| Completeness [%] | 98.4 | 98.4 |
| Redundancy | 6.6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% (v/v) PEG 8000, 100mM Bis-Tris, 200mM magnesium acetate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
