1SLT

STRUCTURE OF S-LECTIN, A DEVELOPMENTALLY REGULATED VERTEBRATE BETA-GALACTOSIDE BINDING PROTEIN

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	59.300, 62.900, 70.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.900
R-factor	0.167
Rwork	0.167
RMSD bond length	0.018
RMSD bond angle	3.900
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.064 *
Total number of observations	84959 *
Number of reflections	20694 *
Completeness [%]	98.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	N-acetyllactosamine	1 (mM)
3	1	drop	dithiothreitol	2 (mM)
4	1	reservoir	ammonium sulphate	54-58 (%sat)
5	1	reservoir	Tris acetate	100 (mM)
6	1	reservoir	MPD	0.5-2 (%(v/v))