1SLQ
Crystal structure of the trimeric state of the rhesus rotavirus VP4 membrane interaction domain, VP5CT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-08-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.916 |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 244.843, 244.843, 104.778 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.200 |
R-factor | 0.308 * |
Rwork | 0.308 |
R-free | 0.33800 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 24.930 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.270 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.057 | 0.120 |
Number of reflections | 51973 | 3446 * |
<I/σ(I)> | 23.6 | 8.8 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 8.4 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 23 * | ammonium sulfate, MPD, Pipes, Tris, EDTA, sodium azide, benzamidine, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 23 * | ammonium sulfate, MPD, Pipes, Tris, EDTA, sodium azide, benzamidine, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9 (mg/ml) | |
2 | 1 | drop | Tris | 20 (mM) | |
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | sodium azide | 0.02 (%) | |
5 | 1 | drop | benzamidine | 0.1 (mM) | |
6 | 1 | reservoir | ethanol | 6 (%) | |
7 | 1 | reservoir | Tris | 50 (mM) | |
8 | 1 | reservoir | dithiothreitol | 1 (mM) |