1SLL

SIALIDASE L FROM LEECH MACROBDELLA DECORA

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-12-24
Detector	MACSCIENCE
Spacegroup name	P 1
Unit cell lengths	46.364, 69.275, 72.469
Unit cell angles	113.48, 95.41, 107.31

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.166 *
Rwork	0.167
R-free	0.22600 *
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.007 *
RMSD bond angle	1.640 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.85)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.090
High resolution limit [Å]	2.000	2.020
Rmerge	0.044 *	0.088
Total number of observations	145108 *
Number of reflections	48481 *
<I/σ(I)>	16	13.5
Completeness [%]	96.5	94.2
Redundancy	3	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.3		THE PROTEIN WAS CRYSTALLIZED BY MACROSEEDING IN 20% PEG 6000, 0.25 M NACL, 0.1 M CACODYLATE BUFFER, PH 6.3., macroseeding

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	5 (mg/ml)
2	1	reservoir	cacodylate	0.1 (M)
3	1	reservoir	PEG6000	20 (%)
4	1	reservoir		0.25 (M)