1SLI
LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH DANA
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-05-24 |
Detector | MACSCIENCE |
Spacegroup name | P 1 |
Unit cell lengths | 46.443, 69.516, 72.514 |
Unit cell angles | 113.14, 95.39, 106.87 |
Refinement procedure
Resolution | 6.000 - 2.000 |
R-factor | 0.182 * |
Rwork | 0.167 |
R-free | 0.22800 * |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | NATIVE STRUCTURE |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.610 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.85) |
Refinement software | X-PLOR (3.85) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.043 | 0.248 |
Total number of observations | 72285 * | |
Number of reflections | 53170 | |
<I/σ(I)> | 9.6 | 3.8 |
Completeness [%] | 74.0 | 56.3 |
Redundancy | 1.4 | 1.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.3 | pH 6.3 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 5 (mg/ml) | |
2 | 1 | reservoir | cacodylate | 0.1 (M) | |
3 | 1 | reservoir | PEG6000 | 20 (%) | |
4 | 1 | reservoir | 0.25 (M) |