1SL9
Obelin from Obelia longissima
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-08-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 83.021, 54.341, 52.442 |
Unit cell angles | 90.00, 112.15, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.170 |
R-factor | 0.1628 |
Rwork | 0.157 |
R-free | 0.19550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1el4 |
RMSD bond length | 0.014 |
RMSD bond angle | 0.032 |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.210 |
High resolution limit [Å] | 1.170 | 1.170 |
Rmerge | 0.045 | 0.249 |
Number of reflections | 61453 | |
Completeness [%] | 84.7 | 65.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | PEG 8000, potassium phosphate, hexaminecobaltic chloride , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |