1SL7
Crystal structure of calcium-loaded apo-obelin from Obelia longissima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.641, 58.641, 110.378 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.990 - 2.200 |
| R-factor | 0.18856 |
| Rwork | 0.186 |
| R-free | 0.23938 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jf2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.307 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.074 | 0.251 |
| Number of reflections | 18694 | |
| <I/σ(I)> | 20.4 | 4.7 |
| Completeness [%] | 99.4 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | modified microbatch | 7.5 | 277 | 20% w/v polyethylene glycol 10000, 0.1M HEPES, pH 7.5, modified microbatch, temperature 277K |
