1SL5
Crystal Structure of DC-SIGN carbohydrate recognition domain complexed with LNFP III (Dextra L504).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-22 |
| Detector | ADSC |
| Wavelength(s) | 1.08940 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 72.570, 55.370, 29.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.020 - 1.800 |
| Rwork | 0.179 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 12903 | |
| Completeness [%] | 93.9 | 88.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% PEG 4000, 0.2M MgCl2, 0.1M Tris pH 8.5. Protein solution: 10 mg/ml protein, 5mM CaCl2, 10mM oligosaccharide., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
