1SKY
CRYSTAL STRUCTURE OF THE NUCLEOTIDE FREE ALPHA3BETA3 SUB-COMPLEX OF F1-ATPASE FROM THE THERMOPHILIC BACILLUS PS3
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-05-24 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 159.500, 159.500, 159.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 3.200 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MITOCHODRIAL F1-ATPASE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 24.650 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.075 | 0.440 |
| Total number of observations | 109106 * | |
| Number of reflections | 22429 * | |
| <I/σ(I)> | 8.9 | 1.5 |
| Completeness [%] | 99.0 | 98.1 |
| Redundancy | 4.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 15 * | HANGING DROP WITH 9-11% PEG20000, 0.12 M SODIUM SULFATE AT PH8.0, EQUILIBRATED AGAINST PROTEIN FREE RESERVOIR WITH HIGHER PEG SOLUTION BY 2%. KEPT AT 15DEG., vapor diffusion - hanging drop, temperature 288K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG20000 | 9-11 (%) | |
| 2 | 1 | drop | sodium sulphate | 0.12 (M) | |
| 3 | 1 | drop | Tris-sulphate | 0.05 (M) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | PEG20000 | 11-14 (%) | |
| 6 | 1 | reservoir | sodium sulphate | 0.12 (M) | |
| 7 | 1 | reservoir | Tris-sulphate | 0.05 (M) |
