1SKF

CRYSTAL STRUCTURE OF THE STREPTOMYCES K15 DD-TRANSPEPTIDASE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Spacegroup name	P 21 21 21
Unit cell lengths	46.593, 54.528, 108.697
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.186 *
Rwork	0.183
R-free	0.24100 *
Structure solution method	MIRAS
RMSD bond length	0.015 *
RMSD bond angle	3.000 *
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.400
High resolution limit [Å]	1.750
Rmerge	0.099 *
Number of reflections	23085
Completeness [%]	80.0
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.2	20 *	pH 7.20

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.3 (mg/ml)
10	1	reservoir	dithiothreitol	0.2 (mM)
11	1	reservoir	PEG4000	15 (%(w/v))	can be replaced by PEG8000
2	1	drop	Tris-HCl	50 (mM)
3	1	drop		0.4 (M)
4	1	drop		10 (mM)
5	1	drop	dithiothreitol	0.2 (mM)
6	1	drop	PEG4000	7.5 (%(w/v))	can be replaced by PEG8000
7	1	reservoir	Tris-HCl	50 (mM)
8	1	reservoir		0.4 (M)
9	1	reservoir		10 (mM)