1SJX

Three-Dimensional Structure of a Llama VHH Domain OE7 binding the cell wall protein Malf1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9326
Spacegroup name	P 32 2 1
Unit cell lengths	71.400, 71.400, 74.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.000 - 2.200
R-factor	0.19163
Rwork	0.191
R-free	0.20644
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hcv
RMSD bond length	0.010
RMSD bond angle	1.280
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.042	0.200
Number of reflections	11594
<I/σ(I)>	3.5	3.5
Completeness [%]	99.8	99.8
Redundancy		6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	10% PEG 6K, 100mM Hepes, 5% MPD, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K