1SJV
Three-Dimensional Structure of a Llama VHH Domain Swapping
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.067 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 124.300, 124.300, 124.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.940 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.094 | |
| Number of reflections | 12491 | |
| Completeness [%] | 98.6 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 26-28% PEG 600, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, temperature 293K |
