1SJS

ACCESS TO PHOSPHORYLATION IN ISOCITRATE DEHYDROGENASE MAY OCCUR BY DOMAIN SHIFTING

Experimental procedure

Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1994-10
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	124.500, 77.500, 92.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.420
R-factor	0.181
Rwork	0.181
R-free	0.24800 *
RMSD bond length	0.005
RMSD bond angle	23.200 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.420
Rmerge	0.075
Total number of observations	37773 *
Number of reflections	13753 *
Completeness [%]	69.0
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4		pH 7.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-8 (mg/ml)
10	1	reservoir	dithiothreitol	2 (mM)
11	1	reservoir	Tris-HCl	50 (mM)
2	1	drop		100 (mM)
3	1	drop	HEPES	100 (mM)
4	1	drop	ATP gamma S	0.05 (M)
5	1	drop		0.05 (M)
6	1	drop	dithiothreitol	2 (mM)
7	1	drop		0.01 (%)
8	1	reservoir	PEG4000	29-32 (%)
9	1	reservoir	ammonium acetate	10-90 (mM)