1SJI
Comparing skeletal and cardiac calsequestrin structures and their calcium binding: a proposed mechanism for coupled calcium binding and protein polymerization
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-01 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5621 |
| Spacegroup name | I 4 |
| Unit cell lengths | 145.188, 145.188, 99.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.196 |
| Rwork | 0.192 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.511 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.570 |
| High resolution limit [Å] | 2.400 | 2.450 |
| Rmerge | 0.052 | |
| Number of reflections | 8778 | |
| Completeness [%] | 89.6 | 66.02 |
| Redundancy | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 282 | PEG400 Na citrate DM Tris-HCl, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 282K |
